BIOS242, Week 2-OL

Name:

Lab 2: Bacterial Isolation

Learning Objectives

· Explain the importance of bacterial growth for the investigation of pathological microorganisms

· Use aseptic techniques

· Explain the concept of a single colony

· Perform plate-streaking techniques

· Use selective media for isolation purposes

Techniques in Lab

· Colony screening

· Sterile technique

· Plate streaking

Introduction: Bacterial Isolation Virtual Simulation Lab
In the Bacterial Isolation simulation, you will investigate the cause of a contamination of poultry meat by a dangerous bacteria strain that is resistant to common antibiotics. After taking samples from the chicken farm, you will work in the virtual laboratory to isolate single colonies of the deadly bacteria among a variety of different species. To do so, you will learn how to work under sterile conditions, and you will be able to practice and perfect your plate streaking technique.

Identifying ampicillin resistant bacteria
The premise of the Bacterial Isolation lab is a report of ampicillin resistant bacteria in poultry meat. You will visit the place of origination, a chicken farm, in hopes of identifying the bacteria strain. The first task is to take a sample, which will contain a variety of bacterial strains, and from that, you must identify which strains are resistant to ampicillin by isolating single colonies.

Aseptic technique
In the Bacterial Isolation lab, you will learn how to use aseptic techniques—for example, remembering to turn on the Bunsen burner and sterile their loop in between streaks.

Plate streaking technique
In order to identify the specific bacteria strain, you will have to perform bacterial isolation using the plate streaking technique. There is an unlimited supply of agar plates, giving you the opportunity to practice this technique as many times as you like. Results are given immediately, as opposed to waiting a full 24 hours for incubation, as when performed in real life as opposed to in a virtual simulation. You will also streak a special Salmonella Shigella agar. The Salmonella Shigella agar contains a certain medium that only promotes growth of Gram-negative strains. Each of the different strains will exhibit a certain phenotype when grown on the Salmonella Shigella agar. By using this information you can identify the specific strain that is resistant to ampicillin. The sample will then be sent for further analysis to fully confirm the identity.

Will you be able to complete the task with your knowledge on bacterial isolation and successfully isolate the dangerous bacterial strain?

Questions:

1. Purpose: Please describe in complete sentences and in your own words, the purpose of this experiment.

2. Why is proper aseptic technique important in microbiology?

3. What is the importance of flaming the inoculating loop or needle before
and after each inoculation?

4. If you do not wait 10 – 20 seconds after flame sterilizing the inoculating instruments before obtaining the sample, what might be the consequences?

5. Why is it important to flame neck of the tubes immediately after uncapping and before recapping the tubes?

6. Reflection: Write 5 sentences on what you learned from this simulation, what did you like and what was something that you would not prefer to be in the simulation.

Grading Rubric:

Activity	Deliverable	Points
Document Submission	Complete lab report and answer questions · Purpose (2 point) · Questions (8 points) · Reflection (5 points)	15
Total	Complete all lab	15

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Soap Note 1 is based on the Case Study # 1

1.Templates used from another classes will not be accepted. Student must use the template provided in this class which must clearly contain the progress note (in the Assessment section) of the encounter with the patient ( this section is clearly mark in bold, highlighted  and underlined). No passing grade will be granted if this section is not completed properly. 
2.Follow the MRU Soap Note Rubric as a guide
3.Use APA format and must include minimum of 2 Scholarly Citations.

4.Check for plagiarism,Turn it in Score must be less than 25% or will not be accepted for credit, must be your own work and in your own words. Copy-paste from websites or textbooks will not be accepted or tolerated.
5.The use of tempates is ok with regards to Turn it in, but the Patient History, CC, HPI, The Assessment and Plan should be of your own work and individualized to your made up patient.  

6.The case study does not contain all the information related to the patient, so you must complete each step of the template according to the symptoms, signs, treatment, diagnostic methods, plan, education, etc. according to the patient’s diagnosis.

7. Attached you can find the rubric and SOAP note template. Also you can find one example.

Please see the attachment for the instructions

 

As a baccalaureate-prepared nursing professional what role will you take to advocate for culturally diverse clients in your healthcare practice?

• Sections of the assignment must include:
  Introduction
  Focus points covered in the discussion posts
  Any arguments and rationales for your stance
  Conclusion or Summary
• The components of your APA Assignment includes the following:
  The assignment must be completed on a word document.
  Your APA Assignment must include a title page (Refer to Purdue OWL).
  All in-text citations must be used when paraphrasing or quoting a previous author.
  All references must reflect the in-text citations used.
  All reference sources must be within the past 5 years unless it is the works of a nurse theorist or a significant reference material.
  The length of the assignment must be 750-1000 words. Please pay attention to spelling and grammar. Points are deducted for repeat offenders.
  Upon completion of the assignment, you must submit it via Turnitin.com and please note that your plagiarism score should be no more than 20%. If you score higher, please make adjustments.
  (Please refer to the APA Grading rubric for additional assistance in completing your assignment).
  You may reach out to the College Writing Center for further assistance.